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KMID : 0624620100430020146
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BMB Reports
2010 Volume.43 No. 2 p.146 ~ p.149
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Induction of insulin receptor substrate-2 expression by Fc fusion to exendin-4 overexpressed in E. coli: a potential long-acting glucagon-like peptide-1 mimetic
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Kim Jae-Woo
Kim Kyu-Tae
Lee Chang-Woo
Jang Sei-Heon
Ahn You-Jin
Jeong Hee-Jeong
Jeong Hyeong-Yong
Ryu Seung-Hyup
Lee Seung-Yeon
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Abstract
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Exendin-4 (Ex-4), a peptide secreted from the salivary glands of the Gila monster lizard, can increase pancreatic β-cell growth and insulin secretion by activating glucagon-like peptide-1 receptor. In this study, we expressed a fusion protein consisting of exendin-4 and the human immunoglobulin heavy chain (Ex-4/IgG-Fc) in E. coli and explored its potential therapeutic use for the treatment of insulin-resistant type 2 diabetes. Here, we show that the Ex-4/IgG-Fc fusion protein induces expression of insulin receptor substrate-2 in rat insulinoma INS-1 cells. Our findings therefore suggest that Ex-4/IgG-Fc overexpressed in E. coli could be used as a potential, long-acting glucagon-like peptide-1 mimetic.
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KEYWORD
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Exendin-4, Glucagon-like peptide-1, Insulin receptor substrate-2, Type 2 diabetes
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